Review: Embryonic stem cells as tools for in vitro gamete production in livestock

GOSZCZYNSKI E DANIEL; NAVARRO MICAELA; MUTTO ADRIAN; ROSS PABLO

ANIMAL

CAMBRIDGE UNIV PRESS

Lugar: Cambridge; Año: 2023

1751-7311

The goal of in vitro gametogenesis is to reproduce the events of sperm and oocytedevelopment in the laboratory. Significant advances have been made in the mouse inthe last decade, but evolutionary divergence from the murine developmental programhas prevented replication of these advances in large mammals. In recent years,intensive work has been done in humans, non-human primates and livestock toelucidate species-specific differences that regulate germ cell development, due to thenumber of potential applications. One of the most promising applications is the use ofin vitro gametes to optimise the spread of elite genetics in cattle. In this context,embryonic stem cells have been posed as excellent candidates for germ cell platforms.Here, we present the most relevant advances in in vitro gametogenesis of interest tolivestock science, including new types of pluripotent stem cells with potential forgermline derivation, characterization of the signalling environment in the gonadalniche, and experimental systems used to reproduce different stages of germ celldevelopment in the laboratory.Powered