Derivation of Bovine Primed Embryonic Stem Cells from Somatic Cell Nuclear Transfer Embryos

SOTO, DELIA A.; NAVARRO, MICAELA; ROSS, PABLO J.

METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)

Humana Press Inc.

Año: 2023 vol. 2647 p. 305 - 315

1064-3745

Derivation of bovine embryonic stem cells from somatic cell nuclear transfer embryos enables the derivation of genetically matched pluripotent stem cell lines to valuable and well-characterized animals. In this chapter, we describe a step-by-step procedure for deriving bovine embryonic stem cells from whole blastocysts produced by somatic cell nuclear transfer. This simple method requires minimal manipulation of blastocyst-stage embryos, relies on commercially available reagents, supports trypsin passaging, and allows the generation of stable primed pluripotent stem cell lines in 3–4 weeks.