BECAS
JEREZ Horacio Emanuel
artículos
Título:
Bacterioruberin from Haloarchaea plus dexamethasone in ultra-small macrophage-targeted nanoparticles as potential intestinal repairing agent
Autor/es:
HIGA, LETICIA H; SCHILRREFF, PRISCILA; BRISKI, ANDRES M; JEREZ, HORACIO EMANUEL; DE FARIAS, MARCELO ALEXANDRE; VILLARES PORTUGAL, RODRIGO; ROMERO, EDER LILIA; MORILLA, MARIA JOSE
Revista:
COLLOIDS AND SURFACES B-BIOINTERFACES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2020
ISSN:
0927-7765
Resumen:
Oral administration of antioxidant andanti-inflammatory drugs have the potential to improve the current therapy ofinflammatory bowel disease. Success of oral treatments, however, depends on thecapacity of drugs to remain structurally stable along the gastrointestinaltract, and on the feasibility of accessing the target cells. Deliveringanti-inflammatory and antioxidant drugs to macrophages using targetednanoparticles, could make treatments more efficient. In this work structuralfeatures and in vitro activity of macrophage-targeted nanostructured archaeolipidcarriers (NAC) containing the high antioxidant dipolar C50 carotenoid bacterioruberin(BR) plus dexamethasone (Dex): NAC-Dex, are described. Ultra-small (66 nm), -32mV ζ potential, 1200 µg Dex /ml NAC-Dex, consisted of acompritol and BR core, covered by a shell of sn 2,3 ether linked archaeolipidsand Tween 80 (2: 2: 1.2: 3 % w/w) were obtained. NAC-Dex were extensively capturedby macrophages and Caco-2 cells and displayed high antiinflammatory andantioxidant activities on a gut inflammation model made of Caco-2 cells and lipopolysaccharidestimulated THP-1 derived macrophages reducing 65 % and 55 % TNF-α and IL-8 release, respectively and 60 % reactive oxygen speciesproduction. NAC-Dex also reversed the morphological changes induced byinflammation and increased the transepithelial electrical resistance, partlyreconstituting the barrier function. Activity of BR and Dex in NACDex waspartially protected after simulated gastrointestinal digestion, improving thechances of BR-Dex joint activity. Results suggest that oral NAC-Dex deservefurther exploration as intestinal barrier repairing agent.
