Caracterization in vitro of ceftazidime and vancomycin eyedrops reconstituted in medium different.

VILARRUBI SILVINA; BESSONE CAROLINA; PALMA SANTIAGO; ALLEMANDI DANIEL; QUINTEROS DANIELA

Rosario, Santa Fe

Congreso; 4º Reunión Internacional de Ciencias Farmacéuticas (RICiFa); 2016

Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR - Facultad de Ciencias Químicas, UNC.

Therapeutic failures to treat Pseudomonas Aeruginosa and Staphylococcus Aureus infections of the eye with conventional treatment regimens, as well as the emergence of more resistant kinds of these bacteria, reveals the need about the use of fortified antibiotic eyedrops in subjects who do not respond to conventional treatment modalities, as is usual in longterm medical care. The increasing need for such treatments has led to the recent appearance in the literature of a number of studies examining the in vitro characteristics of fortified antibiotic eye drops. We were focus on study the characterization in vitro of Ceftazidime and Vancomycin eyedrops. Methods: Solutions of Ceftazidime 30 mg/ ml and Vancomycin 30 mg/ ml were reconstituted with water, buffer pH: 6 and pH: 7 respectively and were stored at 24ºC without exposure to light. Also the samples were prepared with one or two drugs. Changes in the pH values, concentration of those drugs and physical characteristics of the solutions were recorded for the next seven days. Results: The Ceftazidime solutions increased the pH in coincidence with the appearance of visual and olfactory signs of degradation. In the solution of Vancomycin in water and pH: 6, the pHs decreased, and at pH: 7 Vancomycin solution precipitates. When these solutions were mixed the precipitation was observed in the entire sample. The Ceftazidime solution wasn?t precipitated at pH: 6 or pH: 7; but Vancomycin solution precipitated at pH:7.Conclusion: With the obtained results , we prepared reconstituted eyedrops that could be used as an alternative for treating ophthalmic resistant infections.