KIR6.2 protein defficency affects normal liver regeneration after partial hepatectomy in mice

LORENZETTI, FLORENCIA; VERA MARINA C.; HEIT BARBINI, FRANCISCO J.; LAUTARO HESSE; CARLA GABRIELA COMANZO; LUCCI, ALVARO; PISANI, GERARDO B.; CEBALLOS, MARÍA P.; QUIROGA, ARIEL D.; ALVAREZ, MARÍA DE L.

Rosario

Congreso; Reunión anual SAFIS 2019 ROSARIO; 2019

Liver regeneration (LR) after two-thirds partialhepatectomy (PH) in rodents has become a usefulparadigm for studying the unique capacity of the organ toregenerate. Many signals come into play to drive normalLR, among them hepatic ATP content needs to beconserved. Kir6.2/K-ATP channels are expressed in the liver and they play critical roles in the cellular responsesfor tissue protection under stress. Previous results fromour group showed that Kir6.2 knockout mice (Kir6.2-/-)displayed a significant diminution in hepatic PCNA andcyclin D1 protein levels at 48 h post-PH, indicating apossible participation of Kir6.2 in LR. Aim: to evaluate therole of Kir6.2 in LR after PH. Methods and Results: MaleC57/B6 wild type (WT) and Kir6.2-/- mice were subjectedto PH. LR was evaluated at the different phases of LR:priming (1, 2 and 3h), proliferation (48 and 96h) andtermination (168 h) phases after PH. Liver to body weightratio (LW/BW) showed a reduction in Kir6.2-/- mice at thestudied times (1h post-PH: WT= 2.04±0.15; Kir6.2-/-=1.61±0.10*; 2h post-PH: WT= 2.16±0.15; Kir6.2-/-=1.70±0.07*; 3h post-PH: WT= 2.11±0.15; Kir6.2-/-=1.58±0.10*; 48h post-PH: WT= 3.10±0.02; Kir6.2-/-=2.55±0.19*; 96h post-PH: WT= 3.58±0.26; Kir6.2-/-=2.62±0.29*; 168h post-PH: WT= 4.82±0.19; Kir6.2-/-=3.89±0.09*; data are expressed as percent values and aremeans ± SEM; n=5; *p