Biomonitoring of glyphosate and AMPA exposure in an agricultural population from the province of Córdoba for risk assessment via estimated daily intake calculation

FILIPPI, IOHANNA; FERNANDEZ, PILAR; GRIMALT, JOAN O.; BUTINOF, MARIANA; FERNANDEZ, RICARDO; MUÑOZ, SONIA EDITH; AMÉ, MARÍA VALERIA

Montevideo

Congreso; SETAC Latin America 15th Biennial Meeting; 2023

Society of Environmental Toxicology and Chemistry (SETAC)

The widespread use of the herbicide glyphosate (GLY) has raised concerns about the possible adverse effects on human health. Gaps in conclusions among international agencies show up the need for new studies conducted to provide relevant information on exposed populations. Biomonitoring is an important tool for the assessment of the internal dose of exposure to pesticides. It involves the determination of pesticides and/or their metabolites in human matrices and is essential to perform risk assessment in different scenarios of exposure. The actual toxicokinetic knowledge refers that most of the absorbed GLY is eliminated mainly as the parent compound in urine. Therefore, biomonitoring is focused on the determination in urine of GLY, and to a lesser extent on its metabolite aminomethylphosphonic acid (AMPA) to perform risk assessment and to evaluate estimated daily intakes (EDI). The obtained EDI will be finally compared with the acceptable daily intake (ADI) established by regulatory agencies. The aim of the study was to perform a risk assessment of the exposure to GLY by using the concentration of GLY and AMPA in the urine of different populations of one of the most important agricultural areas of the province of Córdoba (Argentina) to calculate the EDI and contribute to the risk management. The exposure to GLY was assessed in two populations with different scenarios of exposure: a- terrestrial applicators of pesticides (subjects occupationally exposed to pesticides), and b- a control group (subjects with environmental exposure to pesticides).The sum of the urinary concentration of GLY and AMPA, the urinary volume excreted per person per day of 1.5 L, the recent knowledge of the human urinary excretion rate (UER) of 1 % of GLY, and the individual body weight were used to calculate the EDI. Then, the results of the EDI were compared with the ADI established by several regulatory agencies.The general media and median values of calculated EDI for both groups of subjects were below the 1 % of the lowest ADI (0.5 mg/kg BW /day) established by the regulatory agency European Food Safety Authority. No statistical differences in EDI median values were found between the two groups of subjects. According to the obtained results, the internal dose of exposure to GLY and AMPA does not represent a risk to the health of the population under study.