Cambios en la expresión del Receptor de Andrógenos en folículos ováricos de rata adulta por exposición perinatal a Bisfenol A.

SANTAMARÍA CG; JACOB A; ABUD J.; MUÑOZ DE TORO M; LUQUE EH; RODRIGUEZ HA

San Juan

Congreso; 2 Reunión Conjunta de las Sociedades de Biología de la República Argentina.; 2011

We have previously found that exposure to environmentally relevant doses of BPA during the neonatal period, affect the follicle development in adulthood and the number of embryo implantation sites. The aim of this study was to evaluate whether BPA could be affecting fertility through ovarian dysfunctions. Female pregnant Wistar rats were treated orally from gestational day 9 until weaning with 50μg BPA/kg-day (BPA50), 0.5μg BPA/kg-day (BPA0.5) or vehicle (alcohol). At PND21 the pups were weaned and the ovary was removed at PND90 (N=8). In serial sections, we evaluated the expression of estrogen Receptor alpha(ERa) and beta(ERb), androgen receptor(AR) and p27 by inmuhistochemistry. The expression of ERa, ERb and p27 was unchanged by BPA, whereas the expression of AR showed a decrease in primordial, preantral and antral follicles, with the dose presumed as safe (BPA50). Previous results have shown that fertility is affected by changes in AR expression. Therefore, our results suggest that perinatal exposure to BPA affects fertility through a decrease in AR expression in the ovarian follicles.