Determination of the Fo¨rster Distance in Polymer Films by Fluorescence Decay for Donor Dyes with a Nonexponential Decay Profile

NEDA FELORZABIHI; PABLO FROIMOWICZ; JEFFREY C HALEY; GHASEM REZANEJAD BARDAJEE; BINXIN LI; ENRICO BOVERO; FRANK CJM VAN VEGGEL; MITCHELL A WINNIK

JOURNAL OF PHYSICAL CHEMISTRY B - (Print)

AMER CHEMICAL SOC

Lugar: Washington; Año: 2009 p. 2262 - 2272

1520-6106

Fluorescence resonance energy transfer (FRET) experiments were carried out on three pairs of donor−acceptor dyes in polymer films in which the donor dyes had absorption maxima in the range of 350−450 nm. Two of the donors, a coumarin dye and a naphthalimide dye covalently bound to polystyrene, gave nonexponential decays in the absence of acceptors. The decay profiles could be fitted to a stretched exponential form with a β value on the order of 0.9. We developed equations for analyzing donor fluorescence intensity decay profiles for donor−acceptor mixtures in rigid matrices for the case of donors showing relatively small deviations from exponentiality. To test these equations, we calculate values of the Förster radius (R0FR) from the decay profile data and compare these values to the Förster radius R0SO determined by the traditional spectral overlap method. Agreement between these values validates the methodology developed here for the use of such donor dyes in FRET studies of more complex polymer systems.