Tuning Ultraluminescence properties from the Nanoscale for Neurophotonics applications by Laser Fluorescence Microscopy coupled to Inflow methods

CARINA SALINAS; LUNA R. GOMEZ-PALACIOS; VALERIA AME; DENIS BOUDREAU; A. G. BRACAMONTE

Congreso; 1st Imaging in Neuroscience virtual conference-Andor, an Oxford Instruments Company; 2021

In this presentation it was showed by varying the design of the Nanoarchitecture how it could be controlled Luminescent and Ultraluminescent properties from gold-silica Nanocomposites. These enhanced Luminescence properties were based on Plasmonic effects named Metal Enhanced Fluorescence (MEF) and Enhanced Plasmonics (EP) coupled to MEF. These phenomena were recorded from the interaction of the high Electromagnetic field in the near field of the metallic nanoparticle surface generated by Laser excitation and fluorescent dyes placed at the right distances for optimal enhancements [1]. About the Nanoarchitectures, it was designed variable dimensions of Biocompatible gold Core-shell silica nanoparticles for MEF applications. [2] The Ultraluminescent properties permitted to be applied to single Nano-bacteria Bioassays with Ultraluminescent emissions. [3] Inflow Nano-optics and Biodetection studies were developed as well. [4], [5] Actually, there are experiments focused on Single Neuro-labelling looking for improved imaging. In this presentation it was afforded the discussion in this theme.Literature:[1] D. Brouard; M. Lessard Viger; A. G. Bracamonte, D Boudreau*, Label-free biosensing based on multilayer fluorescent nanocomposites and a cationic polymeric transducer, ACS Nano, 5 (2011) 1888-1896.[2] D. Gontero, A. V. Veglia, A. G. Bracamonte*, D. Boudreau*, Synthesis of Ultraluminiscent gold Core-shell nanoparticles as NanoImaging Platforms for Biosensing applications based on Metal enhanced fluorescence, RSC Adv. 7 (2017) 10252-10258. [3] D. Gontero, M. Rioux, A. V. Veglia, D. Boudreau, A. G. Bracamonte*, Ultraluminescent gold Core@shell nanoparticles applied to individual bacterial detection based on Metal-Enhanced Fluorescence Nanoimaging, J. of Nanophotonics. Special issue Nanoplasmonics for Biosensing, Enhanced Light-Matter Interaction, and Spectral Engineering, 12, 1, 012505 (2018) 1-12.[4] Luna R. Gomez Palacios, A. V. Veglia, A. Guillermo Bracamonte*, ?In Inflow nano-optics from the near-to the far-field detection based on Metal-Enhanced Fluorescence signaling?, Microchemical J., MICROC 10653 (2021) https://doi.org/10.1016/j.microc.2021.106539.[5] D. Gontero, A. V. Veglia, A. G. Bracamonte*, ?In Flow Metal Enhanced Fluorescence for Biolabelling and Biodetection?, Photochemical & Photobiological Sciences, RSC 19 (2020) 1168?1188. DOI: 10.1039/D0PP00145G.