Non-covalent Bioconjugation chemistry of Metallic Nanoplatforms and Biostructures

C. SALINAS; D. GONTERO; A. V. VEGLIA; V. AME; A. G. BRACAMONTE

Simposio; Corona Virus 2020. Webinar series, LabRoots-ACS; 2020

Different materials for Metallic Nanoparticles within reduced interval of sizes could be used to variable Nanomedicine applications. In this direction, as for example it was designed a Enhanced Fluorescent acceptor silver Core-Silica shell Nanoplatform modified with targeted DNA strands. By this manner, after addition of a Fluorescent donor polymer that non-covalent interacted with the covalent grafted DNA on silanized particles, it was obtained a ready Nanoplatform for DNA detection by non-covalent interaction with the complementary DNA strand. The mechanism of detection was based on the Metal Enhanced Fluorescence Microscopy (MEF) of a Fluorescent acceptor silica shell incorporated close to the silver metallic Core. This Enhanced Fluorescence Energy acceptor collected the Enhanced emission from a triplex non-covalent complex of targeted DNA-fluorescent polymer-complementary DNA strand via a Fluorescence Resonance Energy Transference (FRET) coupled to MEF. , , Moreover, non-covalent interactions from host-guest complexes were used as well to control the MEF effect by a switch on/off chemical reaction. The host used was a biocompatible oligomer cyclic glucose macrocycle named β-Cyclodextrin (βCD). The molecular host-guests complexation free in solution with a classical Laser dye as Rhodamine B (RhB) showed diminished emissions by quenching of the excited state; while on modified gold Nanoparticles with short molecular spacers Enhanced emissions were recorded with potential applications in Single Molecule Detection (SMD). The mentioned metallic Cores were in the size intervals within 40-50 nm, with varied silica shell and short molecular spacers. The different chemistries used on particles permitted the non-covalent interaction with DNA and Fluorescent Dyes. However, it should be highlighted the importance of the different chemistries and possible non-covalent interactions between the Nanoparticles and media. In the same way, these types of Enhanced Luminescent Nanoplatforms showed variable interactions with different Bio-membranes. For this reason, due to that it is of our interest the formation of Nano-Biostructures for Biodetection, Biophotonics, Nanomedicine, and Biotechnology , applications it was initiated a Research work related with the deposition of Ultraluminescent Nanoplatforms. In this presentation it was showed and discussed Bioimaging and Biodetection applications based on the tuning of chemical surface Nanoplatforms for non-covalent labelling of Biostructures, such as Bacteria , and Virus. In the same way, targeted chemical modifications of the Biostructures were considered. Finally, potential applications for Corona-Virus Bioimaging were discussed.