Tuning and controlled switch on/off enhanced Luminescence from NanoImaging platforms for in flow Biodetection and Genotyping applications by Laser Fluorescence Microscopy

C. SALINAS; H. GONZALEZ; C. ROBINSON ; A. VEGLIA ; D. BOUDREAU ; A. G. BRACAMONTE

Simposio; Genetics and Genomics 2018. LabRoots-ACS; 2018

In this presentation it was showed by varying the design of the Nanoarchitecture how it could be controlled Luminescent and Ultraluminescent properties from gold-silica Nanocomposites. These enhanced Luminescence properties were based on Plasmonic effects named Metal Enhanced Fluorescence (MEF) and Enhanced Plasmonics (EP) coupled to MEF. These phenomena were recorded from the interaction of the high Electromagnetic field in the near field of the metallic nanoparticle surface generated by Laser excitation and fluorescent dyes placed at the right distances for optimal enhancements [1]. NanoImaging Analysis was done by Laser Fluorescence Microscopy. Applications in Flow methodologies for Biodetection and Genotyping are in progress.About the Nanoarchitectures, it was designed variable dimensions of Biocompatible gold Core-shell silica nanoparticles (Scheme 1) for MEF applications; and silica Nanocomposites composed by gold Multi-cores for EP coupled to MEF. These Nanoparticles showed variable dimensions accompanied with controlled Ultraluminescent properties. Switch on-off control of the enhanced Luminescence generated was showed by simple chemistry reactions [2]. The higher MEF enhancements factors were determined with higher gold core diameters in the 50-40 nm intervals covered with fluorescent silica shells (Inset of Scheme 1) [3]. However improved Resolution from NanoImaging Analysis was recorded from smaller gold Cores. These Nanoarchitectures were applied for Ultraluminescent Bacteria labelling [4]. Then, in order to study the sensitivity for Single Molecule detection (SMD) based on these enhanced phenomena, these Core-shell Nanoarchitectures were grafted with Supramolecular systems that form host-guest complexes with fluorescent dyes [5]. In addition it was begun studies related with the application of these Nanoplatforms as Energy acceptors for molecular detection as well. It is of our interest study of the variation of the Quantum Yield of the donor-acceptor pairs, in order to be applied for improved biomolecular detection based on Fluorescence Energy Transfer (FRET) coupled to MEF. By this manner, with the Nanoarchitectures synthesized and the different Enhanced Luminescence phenomena studied were discussed their applications for design of Nano-Bioarrays for gen detection applications [6]. Scheme 1 shows the synthesis of Luminescent and Ultraluminescent nanoparticles: a) Silica; b) gold multi-cores nanocomposites; c) and d) gold Core-shell nanoparticles. Image insets show Laser Fluorescence Microscopy Nano-imaging of Luminescent Silica Nanoparticles and Ultraluminescent gold Core-shell nanoparticles. Literature[1] D. Brouard; M. Lessard Viger; A. G. Bracamonte, D Boudreau, Label-free biosensing based on multilayer fluorescent nanocomposites and a cationic polymeric transducer, ACS Nano, 5 (2011) 1888-1896.[2] A. G. Bracamonte, D. Brouard, M. Lessard-Viger, D. Boudreau, A. V. Veglia, Nano-supramolecular complex synthesis: switch on/off enhanced fluorescence control an molecular release using a simple chemistry reaction, Microchemical Journal, 128 (2016) 297?304. [3] D. Gontero, A. V. Veglia, A. G. Bracamonte, D. Boudreau, Synthesis of Ultraluminiscent gold Core-shell nanoparticles as NanoImaging Platforms for Biosensing applications based on Metal enhanced fluorescence, RSC Adv. 7 (2017) 10252-10258. [4] D. Gontero, M. Rioux, A. V. Veglia, D. Boudreau, A. G. Bracamonte, Ultraluminescent gold Core@shell nanoparticles applied to individual bacterial detection based on Metal-Enhanced Fluorescence Nanoimaging, J. of Nanophotonics. Special issue Nanoplasmonics for Biosensing, Enhanced Light-Matter Interaction, and Spectral Engineering, 12, 1, 012505 (2018) 1-12.[5] A. V. Veglia, A. G. Bracamonte, Metal Enhanced fluorescence emission and Quenching protection effect with a host-guest Nanophotonic-supramolecular structure, Submitted to Journal of Nanophotonics, Special Section on Nanoscience and Biomaterials in Photonics. Current state: Accepted (2018) Control number JNP 18013SSP.[6] D. Brouard, O. Ratelle, A. Guillermo, Bracamonte, M. St-Louis, D. Boudreau, Direct molecular detection of SRY gene from unamplified genomic DNA by metal-enhanced fluorescence and FRET, Analytical Methods, 5 (2013) 6896 ? 6899.