Synthesis of gold Nanosupramolecular systems based on short molecular spacers and β-cyclodextrins applied for molecular detection by NanoImaging

HUGO E. GONZALEZ; DANNY BROUARD; ALICIA V. VEGLIA; A. GUILLERMO BRACAMONTE

Congreso; Molecular Diagnostics 2017. Virtual Event. Labroots, ACS; 2017

Recently, development of new Core-shell Nanoparticles conformed by a gold metallic core grafted with molecular spacers and supramolecular systems applied to molecular detection based on Metal Enhanced Fluorescence (MEF), was reported . In the present Research work it was synthesized gold nanoparticles (Au NPs) of 40.0 nm grafted with β-cyclodextrins (βCD, cyclic oligosaccharides of seven unities of glucose that form host-guest complexes) applied for detection of xanthene nuclei, Rhodamine B (RhB). In order to do that, many short molecular spacers were used; as propanoditiol (p-dithiol), Poli-(vinil)pirrolidona (PVP) and 3-(aminopropil)siloxano (APS). In presence of Au-pdithiol-βCD and RhB 1nM, it was observed Luminescent nanoparticles by Laser Fluorescence Microscopy. By this manner it was observed quenched (75%) and enhanced (25-30%) luminescence emission by spectrofluorometry depending of the aggregation state of the nanoparticles. Potential applications for molecular detection, single molecule detection (SMD) and NanoImaging will be discussed.