Is violet LED light efficient to treat superficial lesion like a low grade cervical intraepithelial neoplasia?

ETCHEVERRY, MARIA EUGENIA; MIGUEL PASQUALE; MARIO GARAVAGLIA

Nanchang, China

Simposio; 2021 International Symposium on Biomedical Engineering and Computational Biology (BECB 2021); 2021

Nanchang Hangkong University

Photosensitizers utilized in photodynamic therapy (PDT) can be exited with light of any wavelengths matching their absorptionbands. Thus, superficial lesions, such as those involved in cervical intraepithelial neoplasias (CINs) might be treated with alight of shorter wavelength than red despite the smaller penetration depth in tissue. We review basic aspects of PDT andsome investigations related to the use of light-emitting diodes (LEDs) of different wavelength mainly applied to superficiallesions, and highlight the benefits of the use of violet light and cellular spheroids as a PDT dosage model. Our previousresults from spheroids of cervix carcinoma cells, suggest that the violet light from a LED source is appropriate forperforming PDT of neoplastic lesions involving about 300 m in depth. In this work we present a high fluence light sourceemitting at 420 nm and apply it to perform PDT treatment of a HeLa cell tumor model implanted in BALB/c nude mice. Thephotodynamic reaction is inferred from the fluorescence evolution measured at the tumor and the temperature evolution ofthe treated surface. Violet LED light could be a promising alternative for treating CINs involving superficial dysplasia, withreduced side effects.