Lesiones intraepitelales causadas por HPV: DIAGNOSTICO Y TERAPIA FOTODINÁMICA.

ETCHEVERRY, MARIA EUGENIA; PASQUALE, MIGUEL; GARAVAGLIA MARIO; CORTI AGUSTINA

San Miguel de Tucumán

Congreso; RAFA 2016; 2016

Asociación Fisica Argentina

Hyperthermia is a natural phenomenon caused by hypervascularization in neoplasias as well asphotodynamic reactions by activation of photosensitive drugs in cancer diagnostic (PhotodynamicDiagnostic, PDD) and treatment (Photodynamic Therapy, PDT). Both aspects are relevant indiagnostic and therapeutic techniques for HPV lesions proposed in this work. Then, we use InfraredThermography (IRT) to record the selective temperature increase in malignancies and then definebetter the extent of the injury. We also use the IRT during the application of PDT to maintaintemperature increases controlled. When the temperature exceeds a certain threshold the applicationof the excitation is fractionated to enhance the efficiency of the technique.Practical experience motivated us to think about the idea to perform optical biopsies bySpectrofluorometry (SFM) to study the natural fluorescence of health and HPV-affected tissues.Then by comparing their fluorescence spectra we identified the affected area. Besides, theactivation of the photosensitive molecules accumulated in tumor cells before and after performingPDT is also verified by SFM.The results of the optical biopsies are being compared with data obtained from molecular tests(Hybrid Capture CH2) and the "gold standard" (conventional biopsy).Simultaneously, we are conducting research experiences on PDT in cultured HeLa cells usingphotosensitizers (approved by ANMAT and FDA), and comparing lasers and LEDs as excitationsources. The results were transferred to the animal model and employ SFM in loci to verify theefficiency of PDT procedure, which would allow us to consider its use in human treatments at lowercost.