Physical evidence jutifying experimental biological results in Photodynamic Therapy of Cancer

ETCHEVERRY, MARIA EUGENIA; GARAVAGLIA, MARIO; PASQUALE, MIGUEL

Villa Carlos Paz

Congreso; SAB XLII; 2013

Sociedad Argentina de Biofísica (SAB)

In order to verify the efficiency of absorption of radiation emitted by an LED lamp at 637 nm with an average width of 8 nm in certain concentrations of drug, in this paper we present results recorded with a spectrophotometer (Ocean Optics HR2000 + ES) coupled to an optical fiber. From preliminary results performed on cultured HeLa cells (human cervical carcinoma), using a temoporfirin as photosensitizer and the lamp was obtained the proportion of cell death for different drug concentrations (0.05 mg / ml, 0.25 mg / ml and 1μg/ml) and illumination times (3min, 6min and 12min). The absorption spectrum obtained for temoporfirin showed its characteristic peak centered at 650nm.