Role of surface enhanced Raman spectroscopy in detection of Histamina in biological samples

ETCHEVERRY, MARIA EUGENIA; MELISA BEATRIZ NICOUD; DANIEL SCHINCA

CALI, ARMENIA

Simposio; 10th International Symposium on Optics and its Applications (OPTICS-10); 2022

The use of SERS in order to detect low concetration analytes that previously could not bedetected with Raman spectroscopy, offer a non destructive method to gain molecularinformation about biological samples. This technique have the potential to detect molecularchange prior to any morphological change in tissue, biofluids or in vivo diagnostics.Particularly advantageous for diagnostic purpose when the Raman spectroscopy is combinedwith inherent optical and chemical properties of plasmonic nanoparticules[1,3].Theperformance of the SERS-based diagnostic assay mainly depends on quality of plasmonicnanomaterials and the nano-bio funtional interface.The primary goal of studying any biological fluid with Raman spectroscopy is determinationof abnormalities or biochemical changes compared to normal samples, taking into accountthat most of the biological components present a physiological concentration ranging frommmolL -1 to nmolL -1 .In this work different reproducible and practical surfaces were elaborated for optimize theSERS signal, combining silver nanoparticles whose wavelength plasmonic is at 800 nm, withcellulose on aluminum; and measure histamine at different concentrations.Histamine is a biogenic amine that is involved in both physiological processes, such asmodulation of the immune system and neurotransmission, as in those pathophysiologicalincluding allergic reactions, asthma, inflammation and cancer. In this sense numerouspreclinical and clinical trials have shown that the use of histamine in combination withantitumor therapies (radiotherapy, chemotherapy or immunotherapy) can result in a benefit forthe patient [4].