MITOCHONDRIAL FUNTIONALITY AND ROS PRO- DUCTION ARE COMPROMISED IN Arabidopsis thaliana invh MUTANTS

MARTIN, MARIA VICTORIA; BATTAGLIA MARINA; MARTINEZ-NOEL, GISELLE; SALERNO, GRACIELA

Buenos Aires

Congreso; SAIB; 2017

REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS

Sucrose (Suc) plays a central role in plant growth and develop-ment, and in response to environmental stresses. Alkaline/Neu-tral-Invertases (A/N-Invs) (localized in the cytosol, mitochondriaand/or plastids) are sucrose hydrolyzing enzymes with optimal pHbetween 6.8 and 8.0. Currently, there are many questions about therole of the organellar isoforms. Previous studies in A. thaliana sug-gested that two mitochondrial A/N-Invs (isoforms A and C) have sig-ni cant roles in root growth and development, and in abiotic stressadaptation. However, A/N-InvH is a third isoform whose role is stillunknown. In the present work, we showed using uorescence andconfocal microscopy that invh (a mutant lacking A/N-InvH) exhibitslower mitochondrial membrane potential than wild-type plants. Mo-reover, ROS production in invh roots is impaired not only in salt andABA treated plants but also in control conditions when are comparedwith wild-type plants. Taking together our data, we conclude thatA/N-InvH plays a relevant role in mitochondrial functional statusand in ROS production either under control or stress conditions.Supported by CONICET, ANPCyT (PICT 1288), UNMdP (EXA645)and FIBA.