New insights on alkaline/neutral invertases in plants

LECHNER, LEANDRA; BATTAGLIA, MARINA; MARTIN, MARIA VICTORIA; MARTINEZ-NOEL, GISELLE; SALERNO, GRACIELA

Foz do Iguaçu

Congreso; 11th International Plant Molecular Biology (IPMB, 2015); 2015

MYNCYT-CONICET-SBG-CAPES-INTA-FABESP

<!-- /* Font Definitions */@font-face{font-family:Times;panose-1:2 0 5 0 0 0 0 0 0 0;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:Batang;mso-font-alt:바탕;mso-font-charset:129;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:-1342176593 1775729915 48 0 524447 0;} /* Style Definitions */p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";mso-ansi-language:ES;mso-fareast-language:ES;}span.st{mso-style-name:st;mso-style-unhide:no;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;mso-fareast-font-family:Batang;}@page WordSection1{size:612.0pt 792.0pt;margin:72.0pt 90.0pt 72.0pt 90.0pt;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}-->Alkaline/Neutral Invertases(A/N-Invs) are sucrose hydrolyzing enzymes associated mostly with the delivery of cytosolic hexoses intorespiration. Recent studies revealed new location for these proteins (organelarA/N-Invs) and that they play novel roles. Particularly, they were shown to regulate growth, developmentand responses to environmental challenges. Availability of complete plant genome sequences disclosed A/N-Inv multigenefamilies and opened the door for systematic analysis of their metabolicallyimportant members.Here, we report theidentification of full-length cDNAs for the members of the wheat A/N-Inv genefamily. The genes were clustered into 3 homeologous groups. Phylogeneticanalysis confirmed two subfamilies, consistent with the cytosolic and organelarisoforms. Then, we focused our study on the analysis of the expression of thefour cytosolic putative A/N-Inv encoding genes by reversetranscription-polymerase chain reaction (RT-PCR) experiments in seedlings of twocontrasting wheat cultivars [BioInta 1005, a spring (short cycle) cv. andBaguette 31, a winter (long cycle) cv.]. Differential transcriptlevels were shown in leaves and roots of plants submitted to stress conditions (lowtemperature and drought), being highest in the most tolerant cultivar. Our results support the key role of A/N-Invs in theresponse of plants to cope with abiotic stresses. Supported by UNMdP (EXA645), PICT 1288, and FIBA.