Exogenous proteinases recovered from fishery waste as feed supplement for Nile tilapia (Oreochromis niloticus)

RODRIGUEZ YAMILA ELIANA; PEREIRA NAIR DE LOS ANGELES; HARÁN NORA SELMA; MALLO JUAN CARLOS; FERNÁNDEZ GIMENEZ ANALIA VERÓNICA

Londres

Conferencia; 3rd International Conference on Aquaculture & Fisheries; 2016

The main goal of this research was to analyze in vitro compatibility of Nile tilapia (Oreochromis niloticus) digestive proteinases andenzymes recovered from species comprising fishery waste as Pleoticus muelleri, Artemesia longinaris and Patagonotothen ramsayi.It evaluated the use of exogenous enzymes as feeds supplements to increase digestion efficiency in Nile tilapia fingerlings and juveniles(3.5 ± 0.11 g and 11.6 ± 1.5 g, respectively). We successfully have obtained proteinases from fishery waste as source of exogenous enzymes, demonstrating thatproteinases can be easily extracted and employed as food additives to enhance the digestive process. P. muelleri and A. longinaris enzymes had more activity of acid and alkaline proteinases than P. ramsayi. SDS-PAGE gels demonstrated that Nile tilapia digestive proteinases keep their activity when combined with each exogenous proteinase. Additionally, exogenous enzymes varied in their ability to enhance hydrolysis of different feed ingredients. In conclusion, P. muelleri by-products are the best candidates to be employed as feed supplements for Nile tilapia juveniles. Enzymes from this by-product did not affect the activity and integrity of fish digestive enzymes, improved the hydrolysis of several ingredients, and maintained its activity after being exposed to high temperatures and acid pHs. Also we suggest supplementing first food of the day with exogenous enzymes to obtain their maximum activities. Fishery industry waste has a great biotechnological potential as source of proteinases. Our findings areapplicable to other places where O. niloticus is raised utilizing local fishery waste, and also to different cultured species.