BIOCOMPOSITES BASED ON TPP CROSSLINKED CHITOSAN / BACTERIAL CELLULOSE AS A POTENTIAL STRATEGYFOR CIPROFLOXACIN RELEASE

PACHECO G; CACICEDO ML; ISLAN GA; MENEGUIN AB; CASTRO GR; BARUD HS

Araraquara

Simposio; II International Symposium of Medicinal Chemistry and Regenerative Medicine; 2017

Universidad de Araraquara

ABSTRACT:Introductionand Objectives: Bacterial cellulose (BC) presents highcrystallinity, fibers of nanometric size gives it a greater water hold capacityand not contain lignin, pectin and hemicellulose in its structure. The polymerhas been studied, produced and applied in several areas. Chitosan is apolysaccharide obtained from the N-deacetylation of chitin, consisting ofpolymeric (1→4)-linked 2-amino-2-deoxy-β-D-glucopyranose units. Because of thebiocompatibility, non-toxicity, biodegradability, and intrinsic antibacterialproperties,chitosan is considered as a versatile material with potentialbiomedical applications. Therefore, the aim of this work was to use bacterialcellulose crosslinked with sodium tripolyphosphate (TPP) and chitosan loadedwith ciprofloxacin and to evaluate the antimicrobial capacity and the in vitrorelease study of ciprofloxacin. Materials and Methods: The commercial kitLIVE / DEAD BacLight® wereused for microbiological assays and the bacteriaPseudomonas aeruginosa and Staphylococcus.aureuswereincubated at 24 hours to allow biofilm formation. Subsequently,biofilms were completely covered with empty and ciprofloxacin loadedBC/Chitosan films for 10, 30 and 60 min. Controls with untreated bacteria(Live) and HClO treated biofilm (Dead) were performed. Then, were observed in aLeica DM 2500 epifluorescence microscope (Germany) equipped with UV filters(495?505 nm) at 400X to determine the viability of the bacteria.Ciprofloxacinrelease was evaluated in phosphate buffer (10.0mM pH 5,8). Briefly, one film(10 mm) was incubated in 20 mL buffer at 37°C. Samples were taken at differenttimes, andciprofloxacin was measured at the maximum absorbance wavelength (277nm).Results: For both Pseudomonas aeruginosa and Staphylococcus. aureus, a reduction inthe bacterial population was observed after 30 and 60 minutes of contact withthe bacteria, increasing as time passed. The release profile of ciprofloxacinshowed a gradual release in 15 min (37%) and 25 min (52%) until a burst in 50min (80%) and follow constant. After this quickly release, significantpercentages of the amounts of drug released up to 300 min were not observed,suggesting a prolongation of the release, which could be exploited forpathologies in which an initial loading dose is required, followed by maintenanceof the dose of the antibiotic.Conclusions:The rapid release verified by the study suggests that the system provides asufficient amount of drug for its effectiveness, corroborating with theantimicrobial activity test.Funding:This work was supported by Coordenação de Aperfeiçoamento de Pessoal de NívelSuperior (CAPES).