CYTOTOXICITY, ANTIMICROBIAL ACTIVITY AND MORPHOLOGY OF BACTERIAL CELLULOSE WITH CHITOSANE FILM LOADED WITH CIPROFLOXACIN

MARTINS GL; CACICEDO ML; ISLAN GA; DE CARVALHO RA; AMARAL AC; AMARAL CST; CASTRO GR; BARUD HS; PACHECO G

Araraquara

Simposio; II International Symposium of Medicinal Chemistry and Regenerative Medicine; 2017

Universidad de Araraquara

Introduction and Objectives: Treatment of skin lesions is a great clinicalimportance, justifying the high investments in new products that reduce thetime of healing and increase the patient comfort. As a result, differentproducts and patches are market, however, most are imported and make thetreatment expensive. In this context, biopolymers gain prominence in theindustry due to its efficient, abundant and low cost, such as bacterialcellulose and chitosan. In the present work, the objective was to analyze thecytotoxicity, antimicrobial property and morphology of the film produced bybacterial cellulose and chitosan associated with ciprofloxacin.Materials and Methods: Cytotoxicityassay was performed by the MTT reduction method ({3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), which allows measuring the amount of viablecells based on the principle reduction of MTT salt by mitochondrial enzymes toformazan (ISO 10993-5), in human fibroblast cells GM07492.Inhibition halosagainst Pseudomonas aeruginosa and Staphylococcus aureus were determined byusing modified disk diffusion method according to international clinicalstandards (CLSI/NCCLS), replacing disks for empty and ciprofloxacin loaded BC/chitosanfilms in the agar plate surface (Mueller Hinton agar) and inoculated withbacteria (0.5 McFarland scale) at 37°C for 24 hours. SEM experiments werecarried out using samples previously coated with evaporated carbon. The imageswere obtained using the JEOL T-300 microscope operating at 2 kV.Results: Cytotoxicity assaydemonstrated that the negative control group and CB determined the same patternof cell viability, evidencing the absence of toxicity from the extract of theCB membranes analyzed. Ciprofloxacin loaded BC/chitosan samples exhibited asignificant but slight decrease in the metabolic activity of cells. Incontrast, it does not characterize a cytotoxic influence, considering thepercentage of viability exhibited in the analyzes (greater than 80%).Antimicrobialactivity tests, the ciprofloxacin loaded BC/chitosan film demonstrated activityfor both bacteria tested, in evidence against Pseudomonas aeruginosa that showed higher activity than againstStaphylococcus aureus. The results alsoshowed the antimicrobial activity of chitosan against Pseudomonas aeruginosa, evidenced in the small inhibition haloformed when inoculated the BC/chitosan film, withoutciprofloxacin.Analysis of SEM images revealed surface and transversal section morphologyof films. The surface was homogeneous and characteristic of chitosan. From thetransversal sectional images, was observed the chitosan in the center of filmimmersed in the bacterial cellulose membrane, and at the extremities of thefilm, which averaged 83 µm of thick. Conclusions:Generally, the film did not prove to be toxic, in addition, it presentedantimicrobial activity against the tested microorganisms and had itsmorphological structure characterized. Presented research work will open newprospect for the development of composites that could be used as wound dressingand them potential applications in the field of tissue engineering.Funding: Thiswork was supported by Coordenação de Aperfeiçoamento de Pessoal de NívelSuperior (CAPES)