Chitosan-bacterial cellulose patch of ciprofloxacin for wound dressing: Preparation and characterization studies

CACICEDO, MAXIMILIANO L.; PACHECO, GUILHERME; ISLAN, GERMAN A.; ALVAREZ, VERA A.; BARUD, HERNANE S.; CASTRO, GUILLERMO R.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2020 vol. 147 p. 1136 - 1145

0141-8130

Biopolymeric blends based on bacterial cellulose (BC) films modified with low molecular weight chitosan (Chi) were developed for controlled release of ciprofloxacin (Cip). Biophysical studies revealed a compatibleand cooperative network between BC and Chi including deep structural changes in the BC matrix shown by spectroscopic and thermal analyses (SEM, roughness analysis, FTIR, XRD, TGA, mechanical properties and water vapor transmission rate). Incorporation of chitosan to BC matrix generated a thickeningscaffold with high permeability to water vapor from 0.7 to 3.2 g mm/m2 h. Cip loaded onto the BCChi film showed a hyperbolic release profile with a 30% decrease in antibiotic release mediated by the presence of Chi. BC-Chi blend films containing Cip tested against Pseudomonas aeruginosa and Staphylococcus aureus showed a synergic effect of chitosan on Cip antimicrobial activity. Besides, in vitro studies revealed the lack of cytotoxicity of BC-Chi-Cip films in human fibroblasts