Development of biopolymer nanocomposite for silver nanoparticles and Ciprofloxacin controlled release

ISLAN GA; MUKHERJEE A; CASTRO GR

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 72 p. 740 - 750

0141-8130

Screening of biopolymeric gel beads containing Silver NanoParticles (Ag-NPs)stabilized in Guar Gum Alkyl Amine (GGAA) and Ciprofloxacin (Cip) was carried out inorder to obtain a novel nanocomposite with controlled release profile of both antimicrobians.The selected matrix composed of Alginate/High Methoxyl Pectin (HMP)/GGAA (4:4:1) wasable to co-incorporate Ag-NPs and Cip with encapsulation efficiency higher than 70%. SEMimages revealed good cohesivity and compatibility between the biopolymers and the cargos.Beads provided protection against Ag-NPs degradation at acidic pHs and HMP would playeda key role providing hydrophobic regions. While Cip release profile showed a pHindependent difusional process, Ag-NPs release was restricted to matrix erodability. Calciumquelating agents and/or alginate degrading enzymes could modulate the release profile. Thebactericidal activity of beads was tested in liquid medium, showing cooperative effectsbetween the antimicrobials against Pseudomonas aeruginosa, Escherichia coli, Bacilluscereus and Staphylococcus aureus. TEM images confirmed interaction of Ag-NPs withbacterial surfaces followed by membrane damage. Results suggested the nanocompositematrix as a promising system for oral treatment of intestinal infectious diseases caused bymultidrug resistant and unknown microorganisms, since both Cip and Ag-NPs would be ableto reach intestine in the active form.