Alginate Lyase and Ciprofloxacin Co-Immobilization on Biopolymeric Microspheres for Cystic Fibrosis Treatment

ISLAN GA; BOSIO VE; CASTRO GR

MACROMOLECULAR BIOSCIENCE

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2013 vol. 13 p. 1238 - 1248

1616-5187

A new formulation is described based on biopolymeric microspheres containing alginate lyase(AL) and ciprofloxacin (Cip) for sustainable oral delivery in CF patients. Alginate (ALG) andhigh-methoxyl pectin (HMP) are selected as the biopolymers to develop a composite matrix.ALG microspheres coated with HMP and ALG-HMP blend are gelled in water/organic solventsmixtures, obtaining Cip encapsulations from 46.0 to100.0%. ALG-HMP shows a Cip sustainable releaseprofile and is able to encapsulate 90.0% of AL, showing76.0% enzyme activity after release under simulatedintestinal conditions. The developed system is apromising delivery carrier to treat chronic infectionof Pseudomonas aeruginosa and to reduce theviscoelasticity of the mucus accumulated into intestineof CF patients.