Effects of mineral and vitamin supplementation in the oxidative stress biomarker 3-nitrotyrosine in plasma, urine and liver tissue of dairy cattle during the transition period

ANGELI, E.; BARCAROLO, D.; BIANCIOTTI, L.; REY, F.; ORTEGA, H.H.; HEIN, G.J.

Mar del Plata

Congreso; LXV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica.; 2021

Sociedad Argentina de Investigación Clínica

The intensification of milk production has generated a great metabolic demand in the animals causing an increase in the oxidative stress, particularly during the transition period of dairy cattle. Therefore, we aimed to study the concentration of free 3-nitrotyrosine (3-NT) in plasma, urine and liver tissue, as a marker of the oxidative stress, and other metabolic biomarkers in plasma of cows supplemented with vitamins and minerals during the transition period. The supplemented group (SG; n = 11) received subcutaneously a dose of 5 ml of the vitamin supplement ADAPTADOR® Vit and 5 ml of the mineral supplement ADAPTADOR® Min (Biogenesis Bagó, Bs. As.; vitamin A palmitate 3.5% and vitamin E acetate 5%, copper edetate 1%, zinc edetate 4%, manganese edetate 1% and sodium selenite 0.5%) on -60, -30 and 7 days relative to calving. The control group (CG; n = 11) received two injections of 5 ml of 0.9 % sodium chloride. Blood, urine and liver biopsies were sampled at -21, 7 and 21 days relative to calving and to evaluate 3-NT. Also, plasma concentrations of non-esterified fatty acids, beta-hydroxybutyrate, glucose, albumin, cholesterol, bilirubin, aspartate aminotransferase, gamma-glutamyl transferase and liver triacylglycerol content were spectrophotometrically measured. In cows of the SG, 3-NT concentration was greater in plasma (p < 0.05) and lesser in the liver tissue (p < 0.05). In addition, a lesser liver triacylglycerol content and greater glucose concentration was detected in cows of the SG (p < 0.05). Regarding albumin, plasma concentration was greater in cows of the SG (p < 0.05), with a supplementation x time effect (p < 0.05), being particularly greater on day 21 pre-calving and day 4 post-calving. No statistical differences were evidenced in the other studied parameters. These results suggest that mineral and vitamin supplementation could ameliorate the oxidative stress in the liver and the transition of dairy cows.