EVALUATION OF STEROIDOGENIC ENZYMES IN A MODEL OF FOLLICULAR PERSISTENCE IN DAIRY COWS

CATTANEO MOREYRA, M.L.; GAREIS, N.C.; HEIN, G.J.; ANGELI, E.; ORTEGA, H.H.; SALVETTI, N.R.; RODRIGUEZ, F.; REY, F.

Mar del Plata, Buenos Aires

Congreso; Reunión Anual de Sociedades de Biociencias 2022; 2022

Sociedad Argentina de Investigación Clínica (SAIC)

In dairy cows with cystic ovarian disease, steroid hormones and protein levels of steroidogenic enzymes have been reported to be altered. Furthermore, in cows with ovarian follicular persistence, altered concentrations of estradiol in serum and follicular fluid were observed. Therefore, we aimed to evaluate the protein expression of enzymes involved in the synthesis of steroid hormones (StAR, CYP19, CYP17 and 3β-HSD) in ovarian tissue of cows with follicular persistence. Holando Argentino cows with healthy reproductive tract (n=25) were subjected to estrous cycles synchronization with a G6G-Ovsynch protocol. While cows of the control group (CG, n= 5) were only synchronized, cows of the experimental groups with 0 (P0, n=5), 5 (P5, n=5), 10 (P10; n=5) and 15 days of persistence (P15; n=5), were also treated with progesterone intravaginally (P4). Finally, the ovaries were obtained by bilateral ovariectomy. Histological ovarian sections were used for the indirect immunohistochemistry to localize and quantify in situ protein expression of StAR, CYP19, CYP17 and 3β-HSD in persistent and control follicles. Protein expression of CYP17 in theca cells and StAR in granulosa and theca cells was similar in the analysed groups (p>0.05). Lower CYP19 expression was detected in the P5 and P15 groups compared to the control group (p