MONONUCLEAR IMMUNE CELLS DISTRIBUTION IN THE ENDOMETRIUM DURING POSTPARTUM OF DAIRY COWS

SOFÍA, C.; PERALTA, M.B.; ANGELI, E.; STASSI, A.F.; ORTEGA, H.H.; VELÁZQUEZ, M.M.L.

Mar del Plata, Buenos Aires

Congreso; Reunión Anual de Sociedades de Biociencias 2022; 2022

Sociedad Argentina de Investigación Clínica (SAIC)

The processes involved in the resolution of uterine inflammation during the postpartum period are closely related to an enhanced fertility during the subsequent lactational period. However, little is known about the role of immune cell populations in the endometrial remodeling, restoration and clearance during bovine postpartum. The aim of this study was to analyze the endometrial profile of mononuclear immune populations in absence of disease during bovine postpartum, and their possible relation with delayed conception. Endometrial biopsies were taken from twenty multiparous Holstein dairy cows (second to four lactation, at 45 and 60 days in milk (DIM). All dairy cows returned to their normal cycles before 45 DIM. Their voluntary waiting period was 70 DIM and after one they received the first insemination. Histological sections from endometrial biopsies were processed to analyze the cell immunolocation of total T lymphocytes (LT), Tγδ lymphocytes (LTγδ), B lymphocytes (LB) and macrophages (MØ) by indirect immunohistochemistry (IHC). Dairy cows were grouped according to conception parturition interval (CPI) for comparison: CPI fewer than or equal 90 DIM (CPI≤90), CPI between 90 and 120 DIM (CPI90-120) and CPI greater than 170 DIM (CPI≥170). A Generalized Linear Model (GLM) was used to analyze the mononuclear immune cells distribution in the endometrium. Immunostained cells were mainly located in the endometrial stroma. The number of MØ and LB per mm2 at 45 DIM between the groups showed significant differences (P