Phylogenetic determinants of toxin gene distribution in genomes of Brevibacillus laterosporus

TRAVIS GLARE; ABIGAIL DURRANT; COLIN BERRY; LEOPOLDO PALMA; MARSHA ORMSKIRK; MURRAY COX

GENOMICS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2019

0888-7543

Brevibacillus laterosporus is a globally ubiquitous, spore forming bacterium, strains of which have shown toxic activity against invertebrates and microbes and several have been patented due to their commercial potential. Despite the broad range of organisms susceptible to B. laterosporus strains, rRelatively little is known about this bacterium. Here, we examined the genomes of six published and five newly determined genomes of B. laterosporus, with an emphasis on the relationships between known and putative toxin encoding genes, as well as the phylogenetic relationships between strains. Phylogenetically, strain relationships are similar using average nucleotide identity (ANI) values and multi-gene approaches, although PacBio sequencing revealed multiple copies of the 16S rDNA gene which lessensed its utility at the strain level. Based on ANI values, the New Zealand isolates were distant from the other isolates and may represent a new species. While all of the genomes examined shared some putative toxicity or virulence related proteins, many specific genes were only present in a subset of strains.