Sub1, two domains, two functions: transcription initiation versus elongation

PAULA ALLEPUZ(1), ALEJANDRO COLLIN (1), JAIME ALEGRIO LOURO(2), CARLOS FERNÁNDEZ-TORNERO(2) AND OLGA CALVO(1)

Sant Feliu de Guixols

Congreso; "Gene transcription in yeast: From regulatory networks to mechanisms?; 2014

EMBO

We have performed a detailed analysis of the ssDNA binding and C-terminal domains of Sub1 from S. cerevisiae and identified the essential residues involved in Sub1 DNA binding capacity, which are not required during transcription elongation. Additionally, our data indicates that the C-terminus domain of Sub1, the function of which was previously unknown, might be important in promoting Sub1 release from the promoter to facilitate transcription elongation. In summary, Sub1 plays a dual function during transcription, being a pre-initiation factor via its DNA binding domain, and a transcription elongation factor through its C-terminus domain.