Real-time NMR monitoring of biological activities in complex physiological environments.

MARTIN SMITH; CHRISTOPHER MARSHALL; FRANCOIS-XAVIER THEILLET; ANDRÉS BINOLFI; PHILIPP SELENKO; MITSU IKURA

CURRENT OPINION IN STRUCTURAL BIOLOGY

CURRENT BIOLOGY LTD

Lugar: Londres; Año: 2015

0959-440X

Biological reactions occur in a highly organized spatiotemporal context and with kinetics that are modulated by multiple environmental factors. To integrate these variables in our experimental investigations of ´native´ biological activities, we require quantitative tools for time-resolved in situ analyses in physiologically relevant settings. Here, we outline the use of high-resolution NMR spectroscopy to directly observe biological reactions in complex environments and in real-time. Specifically, we discuss how real-time NMR (RT-NMR) methods have delineated insights into metabolic processes, post-translational protein modifications, activities of cellular GTPases and their regulators, as well as of protein folding events.