HYPERTHYROIDISM IMPROVES GLYCEMIA CONTROL IN TYPE 1 DIABETIC NOD MICE

PITTON JA; MOISO A; SÁNCHEZ MB; MICHEL MC; VIRUEL LB2; NEIRA FJ; PIETROBON EO; SOAJE M; FARÍAS H; VALERIO M; ROSALES JA; ACCORINTI C; VALDEZ SR; MACKERN-OBERTI JP

San Juan

Congreso; Sociedad de Biología de Cuyo; 2023

Sociedad de Biología de Cuyo

Hyperthyroidism (HyperT) disturb the global endocrine status, and may affect several tissues such as heart, skin, liver and ovary. Furthermore, an association between the endocrine thyroid status and immune cells function has also been demonstrated. T and B cells express several thyroid hormone receptors suggesting that thyroid hormone may exert different cellular processes inside immune cells. Additionally, some reports suggest that thyroid hormones may activate B and T cells. However, the precise role of HyperT in autoimmune pathogenesis remains unknown. This raises the question of whether experimental HyperT may modulate the progression of autoimmune diseases, specially in Type 1 Diabetes which is one of the most important autoimmune diseases worldwide. Our study aimed to assess the effect of experimental HyperT on the progression of spontaneous Type 1 Diabetes in NOD mice. To this end, 12 weeks old NOD female mice were injected 5 times per week with s.c. 0,25 mg/kg T4 (HyperT) and vehicle for the control group. All mice were euthanized on the 60th day after initiating treatment with T4. Twenty six animals were used in this study. Body weight was measured. Animals were subjected to the intraperitoneal glucose tolerance test by the injection of 1g/kg glucose to all mice with free access to drinking water.. After 1h of the intraperitoneal glucose administration, animals were bled to evaluate glucose levels using the colorimetric enzymatic method. In terms of body weight at the time of euthanasia, HyperT females showed a higher body weight than control females (25,3±0,2 g vs 23,2±0,6 g control females)(p