Capacity of mayoritary compounds of Achyrocline satureioides to protect from the damage induced by Aflatoxin B1 in wistar rats

SABINI M.C.; ESCOBAR F.M.,; CARIDDI L.N.; BAGNIS G.,; COMINI L.,; CANDELA F.,; MAGNOLI A.; BARBERIS C., ; SABINI L.I.; DALCERO A.M.

Mar del Plata.

Congreso; SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC), LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) Y LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE); 2016

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC), LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) Y LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE)

Aflatoxin AFB1 produced by A. flavus and A. parasiticus, is carcinogenic, teratogenic, hepatotoxic, immunotoxic. t is the most important worldwide mycotoxin for its effect on public health and animal productivity. Macela has several scientifically proven medicinal properties, such as antioxidant, antimicrobial. The objective was to evaluate the ability of majority compounds (MC) of A. satureioides to protect of genotoxic and liver damage induced by AFB1 in rats.Wistar rats 200 g in lots of 4 (2 males and 2 females) were used. Animals were inoculated by intraperitoneal injection. Treatments were 1-AFB1 (1 mg/Kg body weight) + Luteolin (L) (2.5 mg/Kg b.w. ); 2-AFB1 1mg/Lg + Quercetin (Q) (2.5 mg/Kg) 3-AFB1 (1mg/kg) + Chlorogenic acid (CHLA) (5 mg/Kg); 1-negative control (saline solution); 5-p ositive control (cyclophosphamide 30 mg/Kg); 6-AFB1 Control: AFB1(1 mg/kg) dissolved in methanol (5%) and saline solution. Animals were sacrificed by cervical dislocation at 24h post-injection. Experiments: 1-micronuclei in mouse bone marrow: Schmidt W. (1975), number of micronuclei in 1000 polychromatic erythrocytes (PCE) and toxicity index were determined. 2- Hystopathology: parts of the organs were pre-served in 10% buffered formaldehyde (pH 7.4). These samples were cut at 4 um thickness and subjected to haematoxylin/eosin staining for microscopic histological. Photomicrographs were taken and analyzed. The results of genotoxicity revealed that AFB1 at 1 mg/Kg showed an index of genotoxicity of 35MN/1000 PCE, showing statistically significant difference with the negative control (p 􀀞0.001), and that genotoxic damage was reversed with treatment of L andCHLA. while, Q did not protect from damage. Hystopathological examination of the livers of AFB1treated group revealed characteristic lesions like slight fat accumulation and swollen cells. The negative control group and the treatments showed a liver with normal appearance. In conclusion, L and CHLA protect genotoxic and hepatotoxic damage induced by AFB1.MC: Majority compounds L: Luteolin: Q: Quercetin; CHLA: Chlorogenic acid