Comparison of two preservation methods in biocompatibility assays.

LACERRA, CAROLINA; COMÍN, ROMINA; CID MARIANA PAULA; LOPEZ LOZANO, TRISTAN; SCHMETS, DIEGO MAURO; IBARRA THEILER, RAÚL; MÁSCOLO, GERALDINE; SOBRERO, CECILIA BEATRIZ; SALVATIERRA, NANCY ALICIA

SAN JUAN

Congreso; XXIII CONGRESO ARGENTINO DE BIOINGENIERÍA Y XII JORNADAS DE INGENIERÍA CLÍNICA.; 2022

Human amniotic membrane is widely used in regenerative medicine (ophthalmology, dermatology, reconstructive surgery, soft tissue augmentation, and dentistry) and tissue engineering. It is an immuno-compatible tissue that has immunomodulatory, antimicrobial and no mutagenic effects and is also a source of growth factors and cells with stem cell properties that combines adequate mechanical properties with a good capacity for cell adhesion for to the structural components of the extracellular matrix. Several methods are currently used to prepare, store, and sterilize human amniotic membrane allografts that may affect safety and biological properties. The aim of this work was to obtain, at Banco de Tejidos del Laboratorio de Hemoderivados, Universidad Nacional de Córdoba, human amniotic membrane allografts preserved by two methods, cryopreservation and the combination of lyophilization and irradiation. Subsequently, biocompatibility was evaluated using the cytotoxicity, direct irritation and systemic toxicity tests recommended by ISO 10993:5, 10993:10 and 10993:11, respectively. The results showed that the processing method and the different methods of conservation of the amniotic membrane (cryopreserved and lyophilized and irradiated) were adequate. In the cytotoxicity test, both amniotic membranes, showed cell viability greater than 70% according to ISO 10993:5. In addition, no negative evidence was observed in the primary irritation score on the skin, nor there were changes in body temperature due to the systemic administration of the extracts at the different times tested. The allografts obtained and preserved both by cryopreservation and by the combination of lyophilization and irradiation reveal a good profile that makes their use in regenerative medicine and tissue engineering very promising.