INVESTIGADORES
PICCININI Esteban
congresos y reuniones científicas
Título:
Lectin-modified surfaces for the real-time determination of Bone Alkaline Phosphatase by Surface Plasmon Resonance (SPR) Spectroscopy
Autor/es:
SAPPIA, LUCIANO D.; PICCININI, ESTEBAN; MARMISOLLÉ, WALDEMAR; MADRID, ROSSANA E.
Lugar:
Buenos Aires
Reunión:
Workshop; 1st Argentine-German Workshop on Nanotechnology and Nanobiosensors; 2017
Institución organizadora:
INTI
Resumen:
The biological interaction of lectins and carbohydrates rules many recognitions processes found in living organisms [1]. These interactions can be exploited to develop highly stable lectin and glycoprotein biointerfaces [2,3]. In this work, we constructed carbohydrate layers on gold substrates for the immobilization of the lectin wheat germ agglutinin (WGA). The WGA-modified surfaces were used as arecognition element of glycosylated proteins present in human serum. The lectin-glycoprotein recognition event was evaluated by Resonance Plasmon Spectroscopy (SPR), which has a resolution in the subnanometric scale [4].
