Comparative oil extraction from mutt (Myliobatis goodei) liver by enzymatic hydrolysis: free versus immobilized biocatalyst

ANDRÉS H MORALES; JOSÉ H PISA; MARÍA I GÓMEZ; CINTIA M ROMERO; MARINA VITTONE; AGUEDA E MASSAC; DANIELA L LAMAS

Journal of the Science of Food and Agriculture

John Wiley and Sons Ltd

Año: 2023 vol. 104 p. 2493 - 2501

BACKGROUND: The development and fine-tuning of biotechnological processes for fish oil extraction constitute a very importantfocus to contribute to the development of a food industry based on fish consumption. This work lies in a comparative analysisof the oil extraction yield of Myliobatis goodei livers using free and immobilized enzymes.RESULTS: An immobilized biocatalyst was designed from the cell-free extract of a Bacillus sp. Mcn4. A complete factorial designwas used to study the components of the bacterial culture medium and select the condition with the highest titers of extracellularenzymatic activities. Wheat bran had a significant effect on the culture medium composition for enzymatic production.The immobilized biocatalyst was designed by covalent binding of the proteins present in the cocktail retaining a percentageof different types of enzymatic activities (Mult.Enz@MgFe2O4). Among the biocatalyst used, Alcalase® 2.4 L and Purazyme®AS 60 L (free commercial proteases) showed extraction yields of 87.39% and 84.25%, respectively, while Mult.Enz@MgFe2O4achieved a better one of 89.97%. The oils obtained did not show significant differences in their physical–chemical propertieswhile regarding the fatty acid content, the oil extracted with Purazyme® AS 60 L showed a comparatively lower proportionof polyunsaturated fatty acids.CONCLUSIONS: Our results suggest that the use of by-products of M. goodei is a valid alternative and encourages the use ofimmobilized multienzyme biocatalysts for the treatment of complex substrates in the fishing industry.© 2023 Society of Chemical Industry.