THE BISPHOSPHONATE ALENDRONATE EXERTS A DIRECT ACTION ON ADIPOSE TISSUE IN A MODEL OF HYPOESTROGENISM AND OBESITY

CUTINI, P.; CEPEDA, S.; SANDOVAL, MJ.; MASSHEIMER, V.

Mar del Plata

Congreso; REUNION ANUAL DE SOCIEDADES DE BIOCIENCIAS; 2023

SAIC

The expansion of adipose tissue plays a crucial role in the progression of obesity, a chronic disease that generates oxidative stressand an imbalance in the production of proinflammatory adipokinespromoting a deleterious impact in many tissues. Bisphosphonatesare drugs employed as first line therapy for bone-related diseases, such as postmenopausal osteoporosis. However, several extraosseous effects have been demonstrated in the last decades.Postmenopausal women exhibit a higher prevalence of obesity andoverweight, so that, in this work we investigated the impact of thebisphosphonate alendronate (ALN) on adipose tissue in a modelof hypoestrogenism and obesity. For this purpose, retroperitonealadipose tissue was isolated from bilaterally ovariectomized Wistarrats fed with a high-fat diet (27%). For in vitro treatments, slicesof adipose tissue (40 mg) were incubated with 5 μM ALN for 18 h.Control group received vehicle only (phosphate buffered saline). Hydrogen peroxide (H2O2), TBARS and leptin levels were measured.Firstly, we detected that serum H2O2, TBARS and leptin levels inobese rats were higher than those detected in normal weight rats(6, 65 and 122% above control, respectively, P