Bone vascular actions of the nutraceutical Genistein

CEPEDA, S.; SANDOVAL, M.; MASSHEIMER, V.

Mar del plata

Congreso; Reunión Científica Anual SAIC; 2017

Previously we reported that the phytoestrogen (PE) Genistein(Gen) prevents the atherosclerotic plaque genesis via estrogenreceptor (ER) activation and the inhibition of the cellular/molecular events involved in vascular damage. Indeed, vascular musclecell transdifferentation into osteoblasts (OB) like cells was also impaired. Here we studied the role of Gen on the bone-vascular axisinteraction with focus on OB growth and angiogenesis. To that end,aortic rings (AR), primary cultures of aortic endothelial cells (EC)and calvaria OB isolated from female Wistar rats, exposed to Gen(10nM-1uM) were employed. OB proliferation and differentiation areregulated by several factors such as BMP-2, Runx2 and the bioactive compound NO. We showed that short term exposure of OB toGen enhanced NO production (33-20% above C, 15-30 min treatment, p