Role of phytoestrogen Genistein on cellular events involved in bone remodeling: bone-vascular cells crosstalk

SANDOVAL MJ; CAMPELO, AE.; CEPEDA SB; MASSHEIMER VL

Congreso; 2020 Annual Meeting of the American Society for Bone and Mineral Research; 2020

American Society for Bone and Mineral Research

Phytoestrogens (PE) as Genistein (Gen) are proposed as natural therapy for bone lossprevention. We studied the mechanism of action of Gen on osteoblast (OB) differentiation;and on bone-vascular cells interactions with focus on angiogenesis. Aortic rings (AR, exvivo assays) or murine primary cell cultures (in vitro assays) of aortic endothelial cells(EC) or calvarial OB exposed to 10 nM Gen were employed as experimental models. Using RT-PCR technique, we found that Gen increased estrogen receptor alpha (ERα) andRunx2 mRNA levels at 3-5 days of culture (2; 20 fold a/c, respectively, p