Bone cellular actions of Genistein (Gen) involves endothelial cells activation via nitric oxide pathway

CEPEDA, S.; RAUSCHEMBERGER, M.B.; SANDOVAL, M.; MASSHEIMER, V.

Mar del Plata

Congreso; LX Reunión Científica Anual SAIC (Sociedad Argentina de Investigación Clínica); 2016

Sociedad Argentina de Investigación Clínica

Previously we showed that, Gen enhances endothelial cells (EC) proliferation and nitric oxide (NO) synthesis, through a mechanism of action that involves the estrogen receptor. In this work, we studied the effect of Gen on bone-vascular axis and, its relationship with osteoblastic (OB) differentiation. To that end, primary cultures of murine (Wistar rats) endothelial cell (EC) or calvarial preosteoblast cell (OB) were used. OB monolayers were incubated (24 h) with culture medium obtained from EC (conditioned medium C) and OB proliferation was measured (MTT assay and cell counting). Medium C stimulated OB proliferation (13%, p