“Green methods for the extraction of hidroxytyrosol and luteolin from olive oil by-products and bioacessibility evaluation”

NEULS MAYER MORGANA; ESPINO, MAGDALENA; FERNANDEZ MARÍA DE LOS ANGELES; SILVA, MARÍA F.

Conferencia; 2° European Sample Preparation e-Conference (EuSP2022) and the 1° Green and Sustainable Analytical Chemistry e-Conference (GSAC2022),; 2022

In the last years, the consumption of olive oil has experienced a sharp rise due to its organoleptic and healthy properties. Thus, the wastes and by-products derived from the olive oil production have also increased causing important environmental and economic issues1. Consequently, several valorization strategies of these wastes and by-products have been considered.Olive oil by-products contain valuable bioactive compounds, being the phenolic compounds, such as hydroxytyrosol and luteolin, one of the most relevant due to their biological properties 2,3. Generally, the technologies used for the extraction of bioactive compounds are based on the use of different organic solvents presenting many disadvantages for environment and human health. In this scenario, the aim of the present work was to evaluate different Natural deep eutectic solvents (NADES) as extraction media for biocompounds from olive pomace obtained of different varieties and harvest season. Hidroxytyrosol and luteolin concentration, total phenolic content, anthocyanin and antioxidant capacity were determined in the NADES extracts and compared with organic solvent. Furthermore, in vitro digestion of olive pomace extract based on NADES was performed in order to explore their bioaccessibility..A food-grade NADES composed by lactic acid, glucose and water demonstrated excellent recovery values for Hty, Lut, total anthocyanin and phenols from olive pomace. Among the cultivars and recollection seasons under study, Ara 2- LGH extract presented the highest Hty content. Thus, the bioaccessibility of Ara 2- LGH extract was investigated during sequential in vitro digestion, providing essential knowledge for understanding their biological function. Furthermore, notable recovery indexes (> 75 %) were obtained in luteolin and hydroxytyrosol which could exert its potential health benefits. Therefore, NADES are not only greener and more efficient extraction media, but also a way to enhance the bioavailability of natural active ingredients. Our results support the potential of olive oil byproduct for health and nutrition applications..