Effect of Panax ginseng extract on Staphylococcus aureus invasion into bovine mammary epithelial cells and cytokines production

BECCARÍA C.; SILVESTRINI P; SACCO S.C; LOVATO M; RENNA M.S; CALVINHO L.F; BARAVALLE C; DALLARD B.E

Mar del Plata

Congreso; LXI Reunión Cientifica Anual de la Sociedad Argentina de Investigación Clínica- SAIC y LXIV Reunión Anual de la Sociedad Argentina de Inmunología SAI; 2016

Sociedad Argentina de Investigación Clínica

Effect of Panax ginseng extract on Staphylococcus aureus invasion into bovine mammary epithelial cells and cytokines production Beccaria C1, Silvestrini P1, Sacco S1, Lovato MB, Renna MS1, Calvinho LF2, Baravalle C1, Dallard BE1. 1. Laboratorio de Biología Celular y Molecular Aplicada. Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral) (UNL-CONICET). 2. EEA Rafaela, INTA. Panax ginseng extract (PGe) has been widely used as an herbal remedy for various disorders in humans and animals. The aim of this study was to evaluate the effect of PGe on S. aureus invasion into bovine mammary epithelial cells (MAC-T) and quantify the inflammatory response. The MAC-T cells viability treated with different concentrations of PGe (0, 0.5, 1 and 3 mg/ml) was measured by XTT assay. Then, supernatants from MAC-T cultured in 24-well plates with or without different concentrations of PGe were employed for cytokines ELISA test. For S. aureus invasion assays, MAC-T were cultured with PGe (0, 0.5, 1 and 3 mg/ml) before infecting with a S. aureus strain isolated from bovine mastitis, with a multiplicity of infection (MOI) 100:1 bacteria/cell. After challenged with S. aureus for 2 h the MAC-T were washed and treated with gentamicin for 2 h to eliminate extracellular bacteria. Supernatants were then collected and cultured to verify killing by gentamicin. Data were expressed as log10 of colony forming units (CFU)/ml of internalized bacteria. No cytotoxic effects of PGe on MAC-T viability were observed. The highest concentration of PGe (3 mg/ml) induced an increase in the IL-4 production compared with other concentrations and control after 24 h of treatment (p