?GPR30 activation is required for aldosterone-induced non-genomic stimulation of cardiac sodium/bicarbonate cotransporter?.

DE GIUSTI VC; ORLOWSKI A; CIANCIO MC; GONANO LA,; VILA PETROFF MG; AIELLO EA.

Conferencia; Gordon Researches Conferences: Cardiac Regulatory Mechanismis; 2014

GPR30 activation is required for aldosterone-induced non-genomic stimulation of cardiac sodium/bicarbonate cotransporter. The relevance of the rapid non-genomic effects of aldosterone (aldo) in the heart is increasingly appreciated. Through the activation of the classic mineralocorticoid receptor (MR), it has been demonstrated that aldo increases the reactive oxygen species (ROS) production and triggers the activation of the sodium/proton antiporter (NHE) in a non-genomic pathway. However, in the past year, it was proposed that the activation of the novel G protein-coupled receptor 30 (GPR30) mediates the non-genomic effects of aldo in vascular muscle. The aim of this study was to elucidate if the sodium/bicarbonate cotransporter (NBC) is stimulated by aldo and if the activation of GPR30 could mediate this effect. NBC activity was evaluated in rat cardiomyocytes perfused with HCO3-/CO2 solution in the continuous presence of HOE 642 (NHE blocker) during recovery from acidosis (double NH4+ pulse, expressed as percentage of H+ flux (JH) at pHi 6.8 between the 2nd and the 1st pulse) using intracellular fluorescent measurements of BCECF-AM. *