PERSONAL DE APOYO
CIANCIO Maria Carolina
artículos
Título:
Characterization of the Na/HCO3(-) cotransport in human neutrophils.
Autor/es:
GIAMBELLUCA MS*; CIANCIO MC*; ORLOWSKI A; GENDE OA; POULIOT M ; AIELLO EA.
Revista:
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY : INTERNATIONAL JOURNAL OF EXPERIMENTAL CELLULAR PHYSIOLOGY, BIOCHEMISTRY, AND PHARMACOLOGY.
Editorial:
KARGER
Referencias:
Lugar: Basel; Año: 2014 p. 982 - 990
ISSN:
1015-8987
Resumen:
Abstract
Background: Bicarbonate transport has crucial roles in regulating intracellular pH (pHi) in a
variety of cells. The purpose of this study was to evaluate its participation in the regulation of
pHi in resting and stimulated human neutrophils. Methods: Freshly isolated human neutrophils
acidified by an ammonium prepulse were used in this study. Results: We demonstrated that
resting neutrophils have a bicarbonate transport mechanism that prevents acidification when
the Na+/H+ exchanger is blocked by EIPA. Neutrophils acidified by an ammonium prepulse
showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic
transporters SITS or the Na+/HCO3
- cotransporter (NBC) selective inhibitor S0859, and
abolished when sodium was removed from the extracellular medium. In western blot and RTPCR
analysis the expression of NBCe2 but not NBCe1 or NBCn1 was detected in neutrophils
Acidified neutrophils increased the EIPA-insensitive pHi recovery rate when its activity was
stimulated with fMLF/ cytochalasin B. This increase in the removal of acid equivalents was
insensitive to the blockade of the NADPH oxidase with DPI. Conclusion: It is concluded that
neutrophils have an NBC that regulates basal pHi and is modulated by chemotactic agents.