Deciphering the effect of an oxovanadium(IV) complex with the flavonoid chrysin (VOChrys) in intracellular cell signalling pathways in osteosarcoma cell line

LEON I.E; DIEZ P.G; ETCHEVERRY S.B; FUENTES M

Queretaro

Congreso; Latin American Meeting on Biological Inorganic Chemistry (V LABIC); 2016

Vanadium complexes were studied during recent years to be considered as a representative of a new class of non-platinum metal antitumor agents. However, a few challenges are still remaining for the discovery of new molecular targets of these novel metal based drugs. This research deals with the alterations in intracellular signaling pathways promoted by VO-Chrysin complex on a human osteosarcoma cell line (MG-63). Herein it is reported the relative abundance of 224 proteins which are involved in mostly of the common intracellular pathways altered by VO-Chrysin. Besides, we have evaluated the variation of relative tyrosin-phosphorylation levels on full-length human recombinant (FAK and AKT1) kinases. The results of the differential protein expression levels reveal several proteins altered such as PKB/AKT, PAK, DAPK, Cdk 4, 6 and 7, FADD, JNK, among others. Moreover, cell signaling pathway involved in several altered pathways related to PTK2B, FAK, PKC family, suggesting the important role associated with the antitumor activity of VOchrys. Finally, the effect of this compound on in situ expressed FAK and AKT1 is validated by determining the phosphorylation level which is decreased in the first one and increased in the second one.