Plasmid DNA cleavage and cytotoxicity of VO(ODA) complex on normal and tumor lines in culture

LEÓN I.E ; BUTENKO N; BARAN E; CAVACO I.; ETCHEVERRY SB

Congreso; XLVII Reunión Anual de la Sociedad de Bioquímica y Biología Molecular (SAIB 2011); 2011

Vanadium is a transition metal with chemical, biological and pharmacological interest due to insulin mimetics, antitumoral and osteogenic actions. Nevertheless, vanadium compounds may also show cytotoxic effects mainly due to the generation of oxidative stress and DNA damage. In this work we report the biological effects of a complex of vanadyl (IV) cation with two ligands (oxodiacetate and phenanthroline) of biological interest, on two osteoblastic cell lines, one normal (MC3T3-E1) and the other tumoral (UMR 106). The effects on cell proliferation (crystal violet bioassay), metabolic activity (MTT bioassay), lysosomal activity (neutral red) and morphology were investigated. Besides, we studied the mechanisms involved in the cyto- and genotoxicity (oxidative stress and DNA cleavage). The complex caused an inhibitory effect on both line proliferation at low doses with a stronger effect on MC3T3-E1 (p