Synthesis and characterization of CaCO3 - biopolymer hybrid nanoporous microparticles for controlled release of doxorubicin"

BOSIO V.E; CACICEDO M.; CALVIGNAC B.; LEON I.E.; BEUVIER T; BOURY F; CASTRO GR

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2014

0927-7765

tDoxorubicin (Dox) is a hydrophilic drug extensively used for treatment of breast, lung, and ovarian cancer,among others; it is highly toxic and can cause serious side effects on nontargeted tissues. We developedand studied a hybrid nanoporous microparticle (hNP) carrier based on calcium carbonate and biopoly-mers derivatized with folic acid (FA) and containing Dox as a chemotherapeutic drug model. The hNPswere characterized by X-ray diffraction, and Raman and Fourier transform infrared (FTIR) spectroscopies.The X-ray diffraction patterns of calcium carbonate particles showed about 30?70% vaterite?calcite poly-morphisms and up to 95% vaterite, depending on the absence or the presence of biopolymers as well astheir type. Scanning electron microcopy images revealed that all types of hNPs were approximately spher-ical and porous with average diameter 1?5 m, and smaller than CaCO3microparticles. The presence ofbiopolymers in the matrices was confirmed after derivatization with a fluorescein isothiocyanate probeby means of confocal microscopy and FTIR synchrotron beamline analysis. In addition, the coupling oflambda carrageenan (-Car) to FA in the microparticles (FA?-Car-hNPs) increased the cancer-cell tar-geting and also extended the specific surface area by up to ninefold (26.6 m2g−1), as determined bythe Brunauer?Emmett?Teller isotherm. A nanostructured porous surface was found in all instances, andthe FA?-Car-hNP pore size was about 30 nm, as calculated by means of the Barrett?Joyner?Halendaadsorption average. The test of FA?-Car-hNP anticancer activity on human osteosarcoma MG-63 cellline showed cell viabilities of 13% and 100% with and without Dox, respectively, as determined by crystalviolet staining after 24 h of incubation.