Antioxidant and cytotoxic properties of water-soluble crude polysaccharides from two edible mushroom species of Ramaria

TATIANA MUÑOZ-CASTIBLANCO; LUCÍA SANTA MARIA DE LA PARRA,; ROCIO PEÑA-CAÑÓN; KELLY MUÑOZ-LOSADA,; JUAN CAMILO MEJÍA-GIRALDO; LEÓN IE,; MIGUEL ÁNGEL PUERTAS-MEJÍA

ChemistrySelect

John Wiley and Sons Inc

Año: 2024 vol. 9

Water-soluble crude polysaccharides (WSCPs) were isolatedfrom the fruiting bodies of Ramaria flava (Schaeff.) Quél. (RFCP)and Ramaria cf. aurea (Schaeff.) Quél. (RACP). Species ofRamaria were collected in Boyacá-Colombia. WSCPs wereobtained by hot-water extraction, ethanol precipitation, anddialysis and characterized using UV-Vis spectroscopy, gelpermeation chromatography, high-performance liquid chromatography-diodearray detector, fourier transform infrared andnuclear magnetic resonance spectroscopy. Different assaysevaluated the antioxidant capacity, and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT)-assay wasused to determine cytotoxic effects. Chemical compositionalanalysis revealed that RFCP and RACP are heteropolysaccharideswith molecular weights of 407 and 818 kDa, respectivelycompose mainly of glucose, mannose, galactose, fucose, andglucuronic acid with small amounts of protein and polyphenols.The backbone of RFCP and RACP is composed of β-D-Glcp(4!,!3)-β-D-Glcp-(1!,!2,6)-α-D-Galp-(1!,!3,6)-α-D-Manp(1!.Antioxidant capacity demonstrated that both crudepolysaccharides exhibit reducing power, radical scavengingability, and a protective effect on lipid-peroxidation. MTT assayshowed that RFCP and RACP inhibited the growth of cancercells HCT 116-(colorectal) and MG-63-(bone) in a concentrationdependentmanner. Compared with RFCP, RACP showed betterantioxidant capacity and cytotoxic effects due to differences incomposition, molecular weight, and triple-helical conformation.Overall, WSCPs exhibited a promising biological effect, highlightingthe cultural, nutritional, and medicinal value of wildmushrooms.