Ultra-fast quantitation of voriconazole in human plasma by coated blade spray mass spectrometry

MARCOS TASCON; GERMAN AUGUSTO GOMEZ-RIOS; NATHALY REYES-GARCES; JUSTEN POOLE; EZEL BOYACı; JANUSZ PAWLISZYN

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017

0731-7085

Voriconazole is a triazole broad-spectrum antifungal medication often used to treat fungal infections caused by Aspergillus and Fusarium species. One of the main challenges associated with the implementation of this medication is its narrow therapeutic concentration range, demonstrating toxicity at concentrations above 6 μg/mL and limited efficacy at concentrations below 2 μg/mL. As a result, methodologies which permit the rapid and accurate quantitation of voriconazole in patients are highly desirable. In this work two different approaches based on coated blade spray directly coupled to mass spectrometry (CBS-MS) are introduced; each enabling the quantitation of voriconazole in plasma samples with a simple and fast sample preparation and no chromatographic step. The first approach involves a rapid extraction (1 min) of the target analyte from 300 μL of human plasma using conventional laboratory vessels (e.g. vial, 96-well plate). Alternatively, the second strategy consists of a 2 minute extraction from a plasma droplet (10 μL) placed on the coated area of the blade. Both procedures were successfully validated and good linearity (R2 ≥ 0.998), accuracy (91-122%) and precision (