Trypanosoma cruzi virulence factors for the diagnosis of Chagas´ disease

CHAIN, CECILIA YAMIL; PIRES SOUTO, D.E.; SBARAGLINI, M.L.; LABRIOLA, C.A.; DAZA MILLONE, M.A.; RAMIREZ, E.A.; CISNEROS, JOSE S.; LOPEZ-ALBIZU, C.; SCOLLO, K.; KUBOTA, L.T.; RUIZ, A.M.; VELA, MARIA ELENA

Bath

Conferencia; C3Bio Bath Conference on Biosensors, Bioelectronics and Biodevices C3Bio/IBST Joint Event; 2019

University of Bath

Among Trypanosoma cruzi virulence factors, trans-sialidase and cruzipain were reported as potential tools for the diagnosis of Chagas´ disease. Cruzipain immobilized onto 3- mercaptopropionic covered gold substrate showed high capability to discriminate among infected and non-infected clinical samples in Surface Plasmon Resonance-based immunoassays, reducing the time of analysis and allowing to determine higher serum dilutions as positive samples, compared with ELISA, suggesting that cruzipain is a promising candidate to enhance the desirable properties of a biosensor in terms of the development of point of care devices. Recombinant trans-sialidase that retains the catalytic N-terminal domain did not yield specific immunoassays, finding that can be related to the high dilutions of serum used in the immunoassay that hinders the possibility to detect the specific low titer- antibodies.