Transcriptional control of the low-temperature inducible des gene encoding the D5 desaturase of Bacillus subtilis

AGUILAR PABLO S., LOPEZ PALOMA, AND DE MENDOZA DIEGO.

JOURNAL OF BACTERIOLOGY

American Society for Microbiology

Lugar: Washington DC; Año: 1999 vol. 181 p. 7028 - 7033

0021-9193

The Bacillus subtilis des gene encodes the cold-inducible Δ5 lipid desaturase involved in the formation of unsaturated fatty acids from saturated phospholipid precursors. Here, we describe the expression pattern of the des gene in response to a temperature downshift from 37 to 20°C. We found that the synthesis of des mRNA is undetectable at 37°C but dramatically induced upon the temperature downshift. Decay characteristics of the des transcript as well as the in vivo decay of B. subtilis bulk mRNA were investigated. The results showed that the stability of the des transcript as well as of bulk mRNA lasted substantially longer at 20°C than at 37°C. Functional expression of des at 37°C was achieved by exchanging its promoter with the non-cold shock spac promoter. These data provide the first direct evidence that temperature-mediated control of transcription is the major mechanism regulating the mRNA levels of the B. subtilis desaturase. The present results also demonstrate that the only component of the desaturation system regulated by temperature is the desaturase enzyme.